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KMID : 0350519940470010563
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Journal of Catholic Medical College
1994 Volume.47 No. 1 p.563 ~ p.579
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The Relationship between Proliferating Cell Nuclear Antigen(PCNA) and DNA in the Colorectal Carcinoma and their Role in the Prognosis
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Abstract
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Histopathologic scoring, immunohistochemical staining of PCNA and DNA analysis were performed in paraffin embedded tumros amples from 55 colorectal carcinomas(48 adenocarcinoma, 7 mucinous carcinoma). The ten usual inflammator colonic mucosa and
five
lymph nodes were used as control group in immunohistochemical staining and DNA analysis. PCNA, which expressed in colorectal carcinomas were studied by immunoperoxidase staining and DNA proliferative fractions were calculated by bitmap gating
using
flow
cytometry in S, G2, Mphases. Aneuploidy was determined from the DNA histogram using linear S phase method. Clinicopathological parameters were investigated in the view of prognosis. The clinicopathological parameters accessed were patient's age,
sex,
primary site, histologic type, histologic differentiation, mitotic index and modified Dukes stage. Based on the understanding of various characteristics of tumor cells, the search for biologic factors that may predict prognosis were studied.
@ES The results were as follows:
@EN 1. The patients with high mitosis(p=0.0379), poor histological differentiation(p=0.0555), aneuploidy(P=0.0543) and larger tumor size(P=0.0487) had much worse prognosis.
2. PCNA expression significantly increased in colorectal carcinomas(56.11±12.23%) compared with lower half portion of normal mucosa arcound the carcinomas(28.11±12.52%)(P=0.0001) and compared with adenomas(25.45±7.75%)(P=0.0001).
3. PCNA expression in colorectal carcinoma was significantly high in association with increased mitosis(P=0.0083), high Dukes stage(P=0.0257), aneuploidy(P=0.0173), and poor prognosis(P=0.0074). But there was no difference between prognosis and
PCNA
expression in lower half portion of normal mucosa around the carcinoma
4. There was significant relationship between ploidy and histologic differentiation(P=0.0005).
DNA aneuploidy was higher in the poorer histological differention. There was no significant difference between ploidy and mitosis(X*=5.20, P=0.078), but DNA aneuploidy was increased in mitosis grade Ⅲcompared with grade I and Ⅱ.
5. DNA S-phase fraction was higher in the higher mitosis grade among grade I, Ⅱand Ⅲ(P=0.0454).
6. There was a weak positive correlation between PCNA expression and DNA ploidy(r=0.3495). But, there was no correlation between PCNA expression and S-phase fraction(r=0.1268).
In conclusion, this study showed that various histologic and cytokinetic parameters which obtained from colorectal carcinomas offered prognostic indicators. And we suggest that mitosis, histological differentiation, tumor size, PCNA expression,
DNA
S-phase fraction and ploidy, which may represent the proliferative activity of cells, may play major prognostic factors of colorectal carcinoma.
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